English Nederlands

Department: ..............Water research
Dealt with by: ............J.N.P. Schoon
Our reference: ......... 22100222
Re: ..............................Report research "CoolerClean Rinse"
Your contact: ............ Mr Marcel Steur


Amsterdam, February 25, 2000.

Dear Mr. Steur,
I hereby send you our report regarding the research into the effects of the product "CoolerClean Rinse". The
research as conducted on 17 February at OMEGAM.

Purpose
The purpose of the research was to determine whether the product "CoolerClean Rinse" is effective for sanitizing water coolers.

Research method
Two water coolers that had been in use for a certain period of time (and therefore contained bacteria) were used in the present research. The research method was the same for both coolers (the test was performed on each cooler). The only difference was that on one of the coolers (cooler #2) the air filter was treated with CoolerClean Spray. Within the framework of this experiment this was not important, as it did not have any consequences for the sanitation procedure.

At the beginning of the test, the bottles were removed from the coolers and the reservoirs were drained. Then, from both coolers, a sample was taken from the surface in order to determine the germ count.

After that, the reservoirs in the coolers were sanitized using 25 ml of CoolerClean Rinse (by Hygienic Solutions).

CoolerClean Rinse is a 10% dilution of a 50% hydrogen peroxide solution. Therefore, CoolerClean Rinse contains 5% hydrogen peroxide (50 grams per kg). To sanitize the cooler, 25 ml of CoolerClean Rinse is used = 1.25 grams H2O2. This corresponds with 0.7 grams of hydrogen peroxide per liter of water (most reservoirs can contain about 1.75 liters of water).

After the coolers were sanitized using CoolerClean Rinse by Hygienic Solutions, new samples were taken to determine the germ count.

Results
The results are contained in the table below.

Watercooler 1 Watercooler 2
Sampled germ count before sanitation
k
+100
20-100
Sampled germ count after sanitation
k
0
0


Conclusion
The samples taken before the sanitation contain a large number of bacteria;
After treatment with CoolerClean Rinse, the germ count is down to zero;
After treatment with CoolerClean Rinse, the water coolers are fully sterile.

Hoping to have sufficiently informed you,

I remain,

With kind regards,

J.N.P. Schoon
Chief of the Water Department
February 24, 2000.

Department: ..............Water research
Dealt with by: ............J.N.P. Schoon
Our reference: ......... 22100222
Re: ..............................Report research "CoolerClean Rinse"
Your contact: ............ Mr Marcel Steur


Amsterdam, February 25, 2000.

Dear Mr. Steur,
I hereby send you our report regarding the research into the effects of the product "CoolerClean Rinse". The
research as conducted on 17 February at OMEGAM.

Purpose
The purpose of the research was to determine whether the product "CoolerClean Rinse" is effective for sanitizing water coolers.

Research method
Two water coolers that had been in use for a certain period of time (and therefore contained bacteria) were used in the present research. The research method was the same for both coolers (the test was performed on each cooler). The only difference was that on one of the coolers (cooler #2) the air filter was treated with CoolerClean Spray. Within the framework of this experiment this was not important, as it did not have any consequences for the sanitation procedure.

At the beginning of the test, the bottles were removed from the coolers and the reservoirs were drained. Then, from both coolers, a sample was taken from the surface in order to determine the germ count.

After that, the reservoirs in the coolers were sanitized using 25 ml of CoolerClean Rinse (by Hygienic Solutions).

CoolerClean Rinse is a 10% dilution of a 50% hydrogen peroxide solution. Therefore, CoolerClean Rinse contains 5% hydrogen peroxide (50 grams per kg). To sanitize the cooler, 25 ml of CoolerClean Rinse is used = 1.25 grams H2O2. This corresponds with 0.7 grams of hydrogen peroxide per liter of water (most reservoirs can contain about 1.75 liters of water).

After the coolers were sanitized using CoolerClean Rinse by Hygienic Solutions, new samples were taken to determine the germ count.

Results
The results are contained in the table below.

Watercooler 1 Watercooler 2
Sampled germ count before sanitation
k
+100
20-100
Sampled germ count after sanitation
k
0
0


Conclusion
The samples taken before the sanitation contain a large number of bacteria;
After treatment with CoolerClean Rinse, the germ count is down to zero;
After treatment with CoolerClean Rinse, the water coolers are fully sterile.

Hoping to have sufficiently informed you,

I remain,

With kind regards,

J.N.P. Schoon
Chief of the Water Department
February 24, 2000.

Department: ..............Water research
Dealt with by: ............J.N.P. Schoon
Our reference: ......... 22100222
Re: ..............................Report research "CoolerClean Rinse"
Your contact: ............ Mr Marcel Steur


Amsterdam, February 25, 2000.

Dear Mr. Steur,
I hereby send you our report regarding the research into the effects of the product "CoolerClean Rinse". The
research as conducted on 17 February at OMEGAM.

Purpose
The purpose of the research was to determine whether the product "CoolerClean Rinse" is effective for sanitizing water coolers.

Research method
Two water coolers that had been in use for a certain period of time (and therefore contained bacteria) were used in the present research. The research method was the same for both coolers (the test was performed on each cooler). The only difference was that on one of the coolers (cooler #2) the air filter was treated with CoolerClean Spray. Within the framework of this experiment this was not important, as it did not have any consequences for the sanitation procedure.

At the beginning of the test, the bottles were removed from the coolers and the reservoirs were drained. Then, from both coolers, a sample was taken from the surface in order to determine the germ count.

After that, the reservoirs in the coolers were sanitized using 25 ml of CoolerClean Rinse (by Hygienic Solutions).

CoolerClean Rinse is a 10% dilution of a 50% hydrogen peroxide solution. Therefore, CoolerClean Rinse contains 5% hydrogen peroxide (50 grams per kg). To sanitize the cooler, 25 ml of CoolerClean Rinse is used = 1.25 grams H2O2. This corresponds with 0.7 grams of hydrogen peroxide per liter of water (most reservoirs can contain about 1.75 liters of water).

After the coolers were sanitized using CoolerClean Rinse by Hygienic Solutions, new samples were taken to determine the germ count.

Results
The results are contained in the table below.

Watercooler 1 Watercooler 2
Sampled germ count before sanitation
k
+100
20-100
Sampled germ count after sanitation
k
0
0


Conclusion
The samples taken before the sanitation contain a large number of bacteria;
After treatment with CoolerClean Rinse, the germ count is down to zero;
After treatment with CoolerClean Rinse, the water coolers are fully sterile.

Hoping to have sufficiently informed you,

I remain,

With kind regards,

J.N.P. Schoon
Chief of the Water Department
February 24, 2000.

Department: ..............Water research
Dealt with by: ............J.N.P. Schoon
Our reference: ......... 22100222
Re: ..............................Report research "CoolerClean Rinse"
Your contact: ............ Mr Marcel Steur


Amsterdam, February 25, 2000.

Dear Mr. Steur,
I hereby send you our report regarding the research into the effects of the product "CoolerClean Rinse". The
research as conducted on 17 February at OMEGAM.

Purpose
The purpose of the research was to determine whether the product "CoolerClean Rinse" is effective for sanitizing water coolers.

Research method
Two water coolers that had been in use for a certain period of time (and therefore contained bacteria) were used in the present research. The research method was the same for both coolers (the test was performed on each cooler). The only difference was that on one of the coolers (cooler #2) the air filter was treated with CoolerClean Spray. Within the framework of this experiment this was not important, as it did not have any consequences for the sanitation procedure.

At the beginning of the test, the bottles were removed from the coolers and the reservoirs were drained. Then, from both coolers, a sample was taken from the surface in order to determine the germ count.

After that, the reservoirs in the coolers were sanitized using 25 ml of CoolerClean Rinse (by Hygienic Solutions).

CoolerClean Rinse is a 10% dilution of a 50% hydrogen peroxide solution. Therefore, CoolerClean Rinse contains 5% hydrogen peroxide (50 grams per kg). To sanitize the cooler, 25 ml of CoolerClean Rinse is used = 1.25 grams H2O2. This corresponds with 0.7 grams of hydrogen peroxide per liter of water (most reservoirs can contain about 1.75 liters of water).

After the coolers were sanitized using CoolerClean Rinse by Hygienic Solutions, new samples were taken to determine the germ count.

Results
The results are contained in the table below.

Watercooler 1 Watercooler 2
Sampled germ count before sanitation
k
+100
20-100
Sampled germ count after sanitation
k
0
0


Conclusion
The samples taken before the sanitation contain a large number of bacteria;
After treatment with CoolerClean Rinse, the germ count is down to zero;
After treatment with CoolerClean Rinse, the water coolers are fully sterile.

Hoping to have sufficiently informed you,

I remain,

With kind regards,

J.N.P. Schoon
Chief of the Water Department
February 24, 2000.