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Department: ..............Water research
Dealt with by: ............J.N.P. Schoon
Our reference: ......... 22100222
Re: ..............................Report research "CoolerClean Rinse"
Your contact: ............ Mr Marcel Steur
Amsterdam, February 25, 2000.
Dear Mr. Steur,
I hereby send you our report regarding the research into the effects of the product "CoolerClean Rinse". The active ingredient of the product is 5% hydrogen peroxide.
Objective
CoolerClean Rinse is used to sanitize water coolers. The present research was conducted in order to assess whether CoolerClean Rinse effectively sanitized/sterilized the surface and water of a water cooler. The research consisted of three different concentrations of CoolerClean Rinse and three different contact times per concentration. The method of operation that was used for this experiment is related to the application of the product in practice.
Research method
Four clean and sterile beakers were each filled with one liter of water from a water cooler that contained a considerably high bacterial count. The beakers were coded A, B, C and D. In beaker D (blank version), 1 plastic plate was placed, while in the others three plates were placed. Before the start of this experiment, the plates were sterilized. Subsequently, the beakers were stored at room temperature for 10 days.
After 10 days, beakers A through C were treated with CoolerClean Rinse. This treatment consisted of applying a certain dosage of CoolerClean Rinse to the beaker (A through C, respectively 13 mg/l, 1.3 mg/l and 0.13 mg/l) and swinging the beakers for 5 seconds so as to mix CoolerClean Rinse with the water. Then, from each of the beakers a plate was removed at 1 minute, 2 minutes and 5 minutes, each of which were sampled for an overall general germ count. The plate from the non-treated beaker (D) was also sampled to determine the germ count.. This sample will function as non-treated reference.
After having taken surface samples, water samples were taken from each beaker to determine the germ count.
Results
The results of the research are contained in the tables below.
Table 1:
Results of germ count assessment at 37ºCelcius (98.6ºFahrenheit) (incounts per dislide) using dipslides of plastic plates, after exposure to CoolerClean Rinse.
|
Exposure time |
Beaker A (13 mg/l) |
Beaker B (1,3 mg/l) |
Beaker C (0,13 mg/l |
Beaker D Blank version |
| 1 minute |
1-9 |
+100 |
+100 |
20-100 |
| 2 minutes |
0 |
+100 |
+100 |
|
| 5 minutes |
0 |
+100 |
+100 |
|
Table 2:
Results of germ count assessment at 37_C (98.6_Fahrenheit) (in germs per
ml) from beakers A through D.
| |
Beaker A (13 mg/l) |
Beaker B (1,3 mg/l) |
Beaker C (0,13 mg/l) |
Beaker D Blank |
| Germ count 37_(germs/ml) |
0 |
>1,000 |
>1,000 |
>1,000 |
Conclusion
It is only the plates that were exposed to 13 mg/l of CoolerClean Rinse (beaker A) that show a significantly reduced germ count. After only two minutes of exposure to this concentration, these plates are fully sterile; There is no significant difference between the plates from beakers B, C and the blank version, D; The plates from beaker A did show a germ count after one minute of contact time, however, after two minutes the surface had
been fully sterilized.; The water in beaker A did not contain any germs after treatment with CoolerClean Rinse.. Beakers B through D contained over 1,000 germs per ml; kill all the bacteria in the water; a lower dose will not
achieve the same result; In previous experiments, a low dose of 1 ml per liter of CoolerClean Spray (with 2.5% of hydrogen peroxide instead of 5%) was sufficient to keep tap water sterile. Possibly, water from a cooler (used in this experiment) contains different types of bacteria compared to tap water (used in previous experiments), which may react differently to hydrogen peroxide.
Hoping to have sufficiently informed you,
I reman, with kind regards,
J.N.P. Schoon Chief of the Water Department
February 29, 2000.
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